Gallo Lab

Research on brain cancer epigenomics

University of Calgary
Calgary, AB

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3D genome architecture of cancer stem cells 

3D Genome Architecture_Oct 2018-01One of our projects on 3D genome architecture was recently published in Genome Research. This project was led by Michael Johnston, a postdoc in our lab.

Michael generated very high resolution contact maps of cancer stem cells using in situ Hi-C. We were able to define compartments and domains and to visualize individual chromatin loops linking regulatory regions of the genome with the target gene.

By integrating 3D genome maps and other chromatin and transcriptional datasets that we generated, we were able to dissect precise mechanisms of regulation of gene expression.

Among the main findings, we showed that stem cell genes have a tendency to be in contact with multiple super enhancers concurrently. These arrangements might ensure sustained expression of these key genes.

We also found that the 3D genome of cancer stem cells is shaped in such a way as to enable expression of immune-related genes. Among these genes, we focused on CD276, which has a putative role in suppressing T cell function. We found that, unexpectedly, CD276 has a role in maintaining some key stemness properties in GBM cells. We also deployed an antibody-drug conjugate and showed that targeting CD276 may be an effective strategy to kill cancer stem cells in GBM.

The Johnston et al paper is a great example of epigenetic information being used to identify actionable targets and to propose potential new therapeutic avenues for GBM.

 

Evolutionary trajectories in pediatric GBM

Final_Graphical Abstract_Marco-Our manuscript on genomic evolution in pediatric GBM was accepted for publication in Cancer Research and is now available on the journal website.

This project was led by Mary Hoffman, an MSc student in my lab. Her work showed that pediatric GBM harbors many genetic subclones – basically each tumor contains many mini-tumors, each with their own specific genomes.

In addition, our results indicate that pediatric GBM may be driven by populations of slow-cycling cancer stem cells. Because they do not divide very often, these cancer stem cells may be able to escape chemotherapy and radiotherapy during treatment, and may be responsible to re-grow the tumor at relapse.

Overall, Mary’s work showed that pediatric GBM is a complex ecosystem populated by cells with different genomes and functional properties, clearly challenging long-held views that postulated childhood cancers are relatively homogeneous malignancies.

Future efforts in our lab will be aimed at identifying new therapeutic opportunities that specifically target the slow-cycling cancer stem cell populations.

 

Two preprints from our lab are on bioRχiv

 

Our research interests

Our Lab studies the epigenomes of pediatric and adult brain tumors. Our interest in the epigenome stems from our discovery that chromatin architecture specifies cell behaviour, including the key cancer property known as self-renewal. Self-renewing cells are thought to be the root cause of tumor recurrence, because they can contribute to propagation of the malignant cell population indefinitely.

In our Lab, we use patient-derived primary cultures and xenografts as model systems. These platforms can be used effectively to gain significant new knowledge on tumor biology, and as pre-clinical tools for personalized medicine.

 

Our academic affiliations

Departments of Physiology & Pharmacology, Biochemistry & Molecular Biology

Alberta Children’s Hospital Research InstituteCharbonneau Cancer Institute

Cumming School of Medicine

University of Calgary